Analisis Kualitatif Rhodamin B Pada Selai Strawberry Curah Di Pasar Induk Kajen Dengan Metode Benang Wol Dan Kromatografi Lapis Tipis
DOI:
https://doi.org/10.31941/pmjk.v13i1.3033Abstract
Rhodamine B is one of the synthetic dyes that is prohibited in food. However, this substance is still often added to processed food by some persons. The purpose of adding these dyes is to make processed food more attractive, attractive to consumers, and reduce production costs. Rhodamine B that enters the body can cause irritation of the digestive tract, poisoning, impaired liver function, impaired kidney function, and can cause cancer and death. The Kajen Main Market is a meeting place for sellers and buyers to carry out buying and selling transactions. Bulk strawberry jam can be found or purchased at the Main Kajen market. To protect consumers around the Kajen Main Market, it is necessary to research the Rhodamine B content in the jam sold at that market. This research is included in the descriptive observational with a sample of bulk strawberry jam sold at the Kajen Main Market, Pekalongan Regency. Rhodamine B in the sample can be determined using the Thin Layer Chromatography (TLC) method, and wool yarn. Data analysis for the determination of Rhodamine B content using the TLC method was carried out by comparing the Rf value and the appearance of the color of the spots between the samples and the reference standard. A positive result if the Rf value and spot color of the sample and standard have the same result. The wool thread method is based on the bonding of the Rhodamin B color to the fat-free wool thread which is not washed off by water. In this study, from a total of 6 bulk strawberry jam samples, it was concluded that the results of the negative analysis contained the substance Rhodamine B. It can be concluded that it was negative because even though
the Rf results of the samples and standard standards were the same, the samples did not
show clear and bright colored spots on the silica plate when viewed through visible light and UV light, the Rf results show the same number, it is assumed that there are other additives that affect these results. In addition, the negative results were also confirmed by the wool yarn test, which also showed a negative result because the sample dyes absorbed into the wool fiber fibers could be washed off by water, unlike the reference standard which still left color on the wool fiber fibers and was not washed off by water. It is recommended to carry out further research in order to know the content of preservatives and saccharin in bulk strawberry jam
References
Almatsier, S. (2010). Prinsip Dasar Ilmu Gizi. In Gramedia (Ed.), Gramedia Pustaka Utama (Gramedia P). Gramedia.
Hadriyati, A., Lestari, L., & Anggresani, L. (2021). Analisis Rhodamin B dalam Bolu Kukus yang Beredar di Kota Jambi dengan Metode Spektrofotometri UV-Vis. Jurnal Farmasi Dan Ilmu Kefarmasian Indonesia, 8(1), 16. https://doi.org/10.20473/jfiki.v8i12021.16-21
Rahmantika, P. P., & Widwiastuti, H. (2022). Analisis Rhodamin B pada Selai Warna Merah Tanpa Merek yang Beredar di Kecamatan Magetan Menggunakan Metode Kromatografi Lapis Tipis (KLT) JIFS , Volume 2 , Nomor 1 , Juni 2022 PENDAHULUAN Seiring dengan perkembangan industri , Bahan Tambahan Pangan (B). Jurnal Ilmiah Farmasi Simplisia, 2, 59–69.
Samosir, A. S., Bialangi, N., & Iyabu, H. (2018). Analisis Kandungan Rhodamin B pada Saos Tomat yang Beredar di Pasar Sentral Kota Gorontalo dengan Menggunakan Metode Kromatografi Lapis Tipis (KLT). Jurnal Entropi, 13(1), 4.
Sherly Dawile, Fatimawali, F. W. (2013). Analisis Zat Pewarna Rhodamin B Pada Kerupuk Yang. Pharmacon Jurnal Ilmiah Farmasi UNSRAT, 2(03), 86–90.
Siska Rusmalina, M. anung anindhita. (2015). Identifikasi Rhodamin B Dalam Saus Sambal. Jurnal Ilmu Pengetahuan Dan Teknologi, 29(1), 41–46. https://jurnal.unikal.ac.id/index.php/pena/article/view/364
Zarwinda, I., & Elfariyanti, E. (2020). Analisis Rhodamin B pada Cabai Merah Bubuk yang Dijual di Pasar Beureunun dan Pasar Simpang Peut Nagan Raya Provinsi Aceh. Serambi Saintia : Jurnal Sains Dan Aplikasi, 8(1), 23–29. https://doi.org/10.32672/jss.v8i1.2044
Almatsier, S. 2013. Prinsip Dasar Ilmu Gizi Edisi Revisi. Jakarta: Penerbit Gramedia Pustaka Utama
Badan Standarisasi Nasional. 1992. SNI 01-2895,1992: Cara uji pewarna tambahan makanan. Badan Standarisasi Nasional. Jakarta.
Dawile Sherly, Fatimawali, Frenly Wehantouw. 2013. Analisis Zat Pewarna Rhodamin B Pada Kerupuk Yang Beredar Di Kota Manado. Jurnal Ilmiah Farmasi Vol 2 (3).
Departemen Kesehatan Republik Indonesia. 2012. Peraturan Menteri Kesehatan Republik Indonesia Nomor 033 Tahun 2012 Tentang Bahan Tambahan Makanan. Jakarta
Fadhilah, H., 2015. Identifikasi Rhodamin B pada Lipstik yang Beredar di Pasar Grogolan Kota Pekalongan Secara KLT Karya Tulis Ilmiah. Fakultas Ilmu Kesehatan: Universitas Pekalongan
Hadriyati, A., Lestari, L., dan Anggresani, L. (2021). Analisis Rhodamin B Dalam Bolu Kukus Yang Beredar Di Kota Jambi Dengan Metode Spektrofotometri UV-Vi. Jurnal Farmasi Dan Ilmu Kefarmasian Indonesia Vol 8 (1): (16-21)
Hartini, H., dan Raflesia, S. (2020). Analisis Kandungan Pewarna Rhodamin B Pada Selai Stroberi Yang Dijual Di Pasar Dupa Pekanbaru. Jurnal Sains dan Teknologi Laboratorium Medik Vol 5 (1): (11-15)
Rahmantika, P.P., dan Widwiastuti, H. 2022. Analisis Rhodamin B Pada Selai Warna Merah Tanpa Merek Yang Beredar di Kecamatan Magetan Menggunakan Metode Kromatografi Lapis Tipis (KLT). Jurnal Ilmiah Farmasi Simplisia Vol 2 (1): (59-69)
Rusmalina S., dan Anindhita, M.A. 2015. Identifikasi Rhodamin B Dalam Saus Sambal Yang Beredar Di Kota Pekalongan. Jurnal Online Universitas Pekalongan Vol 29 (1)
Samosir, A.S., Bialangi, N., dan Hendri Iyabu. 2018. Analisis Kandungan Rhodamin B Pada Saos Tomat Yang Beredar Di Pasar Sentral Kota Gorontalo Dengan Mengunakan Metode Kromatografi Lapis Tipis (KLT). Inovasi Penelitian, Pendidikan, dan Pembelajaran Sains. Jurnal Entrop Vol 13 (1): pp (45-49).
Sugiyono. 2018. Metode Penelitian Kualitatif, Kuantitatif, dan R&D. Bandung: Penerbit Alfabeta.
Zarwinda, I., dan Elfariyanti, E. (2020). Analisis Rhodamin B Pada C abai Merah Bubuk Yang Dijual Di Pasar Beureunum dan Pasar Simpang Peut Nagan Raya Provinsi Aceh. Serambi Saintia: Jurnal Sains dan Aplikasi Vol 8 (1): (23-29)
Downloads
Published
Issue
Section
License
Authors who publish with PENA MEDIKA : JURNAL KESEHATAN agree to the following terms:
- Authors retain copyright and grant the PENA MEDIKA : JURNAL KESEHATAN right of first publication with the work simultaneously licensed under Creative Commons Attribution License (CC BY 4.0) that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors can enter into separate, additional contractual arrangements for the non-exclusive distribution of the published version of the work (e.g., post it to an institutional repository or edit it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) before and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
