EFFECT OF ETHANOL CONCENTRATION ON ANTIBACTERIAL ACTIVITY OF PARISIAN LILY (Chlorophytum comosum) LEAF EXTRACT
DOI:
https://doi.org/10.31941/benzena.v4i01.6644Abstract
Staphylococcus aureus is a pathogenic bacterium that frequently causes serious infections, such as skin infections, pneumonia, and sepsis. The increasing resistance to conventional antibiotics has prompted the search for alternative therapies derived from natural sources. Spider plant (Chlorophytum comosum) leaves are known to contain bioactive compounds such as flavonoids, saponins, and terpenoids, which have potential antibacterial properties. This study aims to determine the effect of varying ethanol concentrations (40%, 70%, and 96%) on the antibacterial activity of spider plant leaf extracts against Staphylococcus aureus. Extraction was carried out using the maceration method with ethanol solvents at concentrations of 40%, 70%, and 96%. Each extract underwent phytochemical screening. Antibacterial activity was tested using the well diffusion method against Staphylococcus aureus ATCC 25923. The inhibition zones were measured using a caliper. Data were analyzed using normality and homogeneity tests, one-way ANOVA, and Tukey’s post hoc test. Phytochemical screening results showed that the 40% ethanol extract contained only flavonoids and tannins, while the 70% and 96% ethanol extracts contained flavonoids, terpenoids, tannins, and saponins. Antibacterial testing showed that the 70% ethanol extract produced the largest inhibition zone (9.75 mm), followed by the 96% ethanol extract (7.25 mm), and the 40% ethanol extract (6.3 mm). ANOVA results indicated significant differences between groups (p < 0.05), and the post hoc test showed that the 70% ethanol extract differed significantly from the other two groups. Ethanol 70% was the most effective solvent for extracting antibacterial compounds from spider plant leaves, as it produced the highest antibacterial activity against Staphylococcus aureus. These results support the potential use of spider plant leaf extract as a natural antibacterial candidate.
Downloads
Downloads
Published
Issue
Section
License
uthors who publish with BENZENA Pharmaceutical Scientific Journal agree to the following terms:- Authors retain copyright and grant the BENZENA Pharmaceutical Scientific Journal right of first publication with the work simultaneously licensed under Creative Commons Attribution License (CC BY 4.0) that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors can enter into separate, additional contractual arrangements for the non-exclusive distribution of the published version of the work (e.g., post it to an institutional repository or edit it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) before and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
